triplett@CALSHP.CALS.WISC.EDU (09/24/90)
Hello Bionet friends. I am new to BioNet and to DNA sequencing. I need to analyze a 5 kb sequence of bacterial (Rhizobium) DNA for ORFs, start and stop codons, operons, etc. Are there any DNA sequence analysis programs in the public domain that I can use? GCG programs are not very user friendly and they are expensive to use. DNA* programs are also expensive. I also don't know how good they are. Any suggestions would be greatly appreciated. Eric Triplett Dept. of Agronomy Univ. of Wisconsin-Madison email: triplett@wiscmac3 telephone: 608-262-9824
gilbertd@silver.ucs.indiana.edu (Don Gilbert) (09/25/90)
In article <9009240037.AA12429@calshp.cals.wisc.edu> triplett@CALSHP.CALS.WISC.EDU writes: > >Hello Bionet friends. I am new to BioNet and to DNA sequencing. >I need to analyze a 5 kb sequence of bacterial (Rhizobium) DNA for >ORFs, start and stop codons, operons, etc. Are there any DNA sequence >analysis programs in the public domain that I can use? GCG programs are If you have access to a SUN or other Unix computer, then contact Chris Fields, cfields@nmsu.edu, about his _gm_ program, or see the June? 1990 CABios for an article on gm. His program is easy to use, and is designed to do just as you ask: analyze an unknown stretch of dna for ORFS and such, and it does it in a state of the art fashion using several tests. If you have a computer that emulates XWindows terminal, you can get a nice graphic display. The program is free. -- Don Don.Gilbert@Iubio.Bio.Indiana.Edu biocomputing office, indiana univ., bloomington, in 47405, usa
frist@ccu.umanitoba.ca (09/27/90)
In article <9009240037.AA12429@calshp.cals.wisc.edu> triplett@CALSHP.CALS.WISC.EDU writes: > >Hello Bionet friends. I am new to BioNet and to DNA sequencing. >I need to analyze a 5 kb sequence of bacterial (Rhizobium) DNA for >ORFs, start and stop codons, operons, etc. Are there any DNA sequence >analysis programs in the public domain that I can use? GCG programs are >not very user friendly and they are expensive to use. DNA* programs >are also expensive. I also don't know how good they are. Any >suggestions would be greatly appreciated. > Eric Triplett > Dept. of Agronomy > Univ. of Wisconsin-Madison > email: triplett@wiscmac3 > telephone: 608-262-9824 Try the Fristensky Sequence Analysis Package. These programs are not as fancy, in terms of having graphic user interfaces, as some of the commercial packages, but rather employ system-independent menus which are pretty self-explanatory. In general, but they do most of what you need to do for DNA sequencing. For MS-DOS users, send five 5.25in diskettes to: MBCRR Coordinator Dana Farber Cancer Institute 44 Binney Street Boston, MA 02115 USA Unix users should contact me. =============================================================================== Brian Fristensky | "I'm not just a Jehovah's Witness, I Dept. of Plant Science | also sell insurance." University of Manitoba | Winnipeg, MB R3T 2N2 CANADA | frist@ccu.umanitoba.ca | from DAVID LETTERMAN'S TOP TEN UNPLEASANT Office phone: 204-474-6085 | THINGS TO HEAR ON AN ELEVATOR. FAX: 204-275-5128 | ===============================================================================