GRAZIAWD@SPLAVA.CC.PLATTSBURGH.EDU ("William D. Graziadei") (02/28/91)
Date sent: 27-FEB-1991 20:48:49 In a recent message from Mario, he asked >From: IN%"nenno@rhrk.uni-kl.de" 27-FEB-1991 20:09:17.67 >To: software@rhrk.uni-kl.de >CC: >Subj: Searching for educational software in molbio (gel/fragments) !!! > >I'm looking for a educational program to demonstrate the principle >of agarose gel to display the DNA fragments depending on its molecular weigh. > >The program should run on a PC with Mono or VGA Card and display >the result of a gel as a graphic with bands, that shows how far > ======================================= >several fragments would run on the gel, depending on its molecular weight. >The program will be used in a course in molecular biology to demonstrate >the principle. For example: We have utilized SuperCalc 5 spreadsheet program (which can operate like Lotus as well as export files as Lotus) to perform the kind of data analysis and output you seek. If SC5 or 123 are available to you I would be glad to provide the template files to you. Let me know. The data entry is smple. Measure the distance and score each band, smear, etc on a relative intensity scale of +1, +2, +3, etc. You then enter relative score in the template file at the proper distance. The marker distances and relative intensity are utilized to plot a semi-log output of bases vs distance traveled in cm. The relative intensity of samples is utilized in a graphic output as an electrophoretic profile vs the plot of marker size in bp. This is much less expensive than photographs and the students like it because each can produce their own output for their research book. Hope this helps. Regards, Bill Send EMail replies to Bitnet or Internet address to by-pass the list. +-------------------------------------------------------------------------+ | Voice: (518) 846-7144 | William D. Graziadei, PhD | | Fax: (518) 846-7144 Ext 40 | In Vitro Cell Biology & | | (518) 564-7827 | Biotechnology | | Bitnet: GRAZIAWD@SNYPLAVA | SUNY Plattsburgh/Miner Inst| |Internet: GRAZIAWD@SPLAVA.CC.PLATTSBURGH.EDU| Chazy, New York 12921 | +-------------------------------------------------------------------------+