danielg@uncmed.med.unc.edu (02/01/90)
I have a small technical problem, perhaps one of you can give me a simple
solution.
When I electrophorese my pcr products, I am looking for a band around
200 bases long (pretty small). The problem is that the band usually comes
out right at the dye front, which makes UV visualization a slight problem.
Is there another dye I can use that will run a bit faster or slower than
bromphenol blue? I could try just using glycerol and edta in my loading
buffer, leaving out the dye.
All suggestions welcome...
Daniel G. Sinclair
Rsch Tech II
University of NC, Chapel Hill
Disclaimer: My opinion means | 'If you only knew how much I was holding
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