[triangle.general] visualizing small pcr fragments

danielg@uncmed.med.unc.edu (02/01/90)

I have a small technical problem, perhaps one of you can give me a simple

When I electrophorese my pcr products, I am looking for a band around 
200 bases long (pretty small).  The problem is that the band usually comes
out right at the dye front, which makes UV visualization a slight problem.

Is there another dye I can use that will run a bit faster or slower than
bromphenol blue?  I could try just using glycerol and edta in my loading
buffer, leaving out the dye.

All suggestions welcome...

Daniel G. Sinclair
Rsch Tech II
University of NC, Chapel Hill

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