CABELL.ANDREWS@BIONET-20.BIO.NET (Paul Andrews) (06/15/89)
Protein Isolation:
I am interested in isolating an inner mitichondrial membrane
protein which has been expressed as a fusion protein in an E. coli strain.
Unfortunately, becasue of its extreme lipophilicity, the only way I have been
able to pull it out from the bacteria is with hot SDS resulting in extensive
denaturation of the protein. Anyone have experience in isolating these
type of membrane proteins in an active form from this type of matrix?
--------------------------------------------------------------------------
Paul Andrews ARPANET: CABELL.ANDREWS@BIONET-20.BIO.NET
Univ. of Texas at Austin Phone 512-471-1731
College of Pharmacy
-------