CABELL.ANDREWS@BIONET-20.BIO.NET (Paul Andrews) (06/15/89)
Protein Isolation: I am interested in isolating an inner mitichondrial membrane protein which has been expressed as a fusion protein in an E. coli strain. Unfortunately, becasue of its extreme lipophilicity, the only way I have been able to pull it out from the bacteria is with hot SDS resulting in extensive denaturation of the protein. Anyone have experience in isolating these type of membrane proteins in an active form from this type of matrix? -------------------------------------------------------------------------- Paul Andrews ARPANET: CABELL.ANDREWS@BIONET-20.BIO.NET Univ. of Texas at Austin Phone 512-471-1731 College of Pharmacy -------