BS04%UK.AC.DUNDEE.PRIMEB@CORNELLC.CIT.CORNELL.EDU (05/25/90)
I hope some one can save me reinventing the "wheel". I have recently carried out some native PAGE 7.5% on some crude extracts of Phaeodactylum tricornutum a marine diatom. The crude extracts have low protein levels about 30 ug / 100 uL and staining with CBB just shows 4 bands at the most. The most abundant protein is RUBISCO the main carboxylase. I have thought about silver staining and this is where I need the advice/help. A morning in the library and I came up with Switizer, Merril and Shifren (1979) and Oakley, Kirsh and Morris (1980). The latter appears to be a good modification of the former but reading more recent papers it does not seem to get quoted all that often. We have a Phastsystem for electrophoresis but I would like to some 20x20 cm gels ago and stain with Silver. I would like to know if anyone can recommend the current method for silver staining polyacrylamide gels. Many thanks......... Andy J. Andrew M. Johnston, E-Mail: BS04@PRIMEB.DUNDEE.AC.UK Biological Sciences, Tel No: 0382-23181 Ext 4282 University of Dundee, Dundee Scotland DD1 4HN DUNDEE UNITED GIVE A CHAP A HARD TIME!!!