37_410@uwovax.uwo.ca (09/06/90)
Does anyone have any experience with proteins that migrate significantly slower by SDS-PAGE than their DNA sequence predicts? I have a 22.3 Kd ORF that yields a 35 Kd polypeptide on gels. I would like to know if someone has similar experience or some possible explanation(s). Jim Gordon University of Western Ontario, Ontario, Canada email: (care of) T.LINN@uwovax.uwo.ca