BS04@primeb.dundee.ac.uk (03/15/91)
Networkers, Could I have some advice on Protease inhibitors? I am just about to start using them as part of my extraction buffer. The amounts I need to buy are quite small and for every extraction even less. The question I would like to ask is: Is it possible to make up the solution in the vial the chemical comes in and freeze aliquots? Often when only small amounts of a chemical are obtained a fraction always seems to be left in the lid/sides/bottom of the vial! and quite alot is "lost". The chemicals I intend to use are (1) Aprotinin (2) Leupeptin (3) Pepstatin If anyone has any alternatives/recommendations on the use of these chemicals I would be more than grateful. Yours Andrew Johnston. Andrew M. Johnston, E-Mail: BS04@PRIMEB.DUNDEE.AC.UK Biological Sciences, Tel No: 0382-23181 Ext 4282 University of Dundee, Dundee Scotland DD1 4HN