BIO252@YaleVM.YCC.Yale.Edu (01/11/89)
In a paper by DeSalle et al. (1987; Genetics 116:215-223) a method is proposed to assess heterogeneity between populations using mtDNA haplotypes using arsin, square-root transformed frequency of a given haplotype in the following statistic: V = 4 x summation ni *((ai - mean a) squared) where ni = the number of individuals in the ith sample, ai = the number of individuals in the ith pop. with the haplotype of interest, mean a = sum ni x ai/N, N = total number of individuals in all pop. and r = the number of populationns. Under the null hypothesis of no geographical heterogeneity, V is distributed as a Chi-Square with r-1 degrees of freedom. The significance of Fst values are tested with the V statistic. In examples with two haplotypes, alternate haplotypes give similar V values. But where several haplotypes occur, it appears that V values calculated for any one of them may underestimate or over estimate between population heterogeneity. Any suggestions on how to alter this statistic to give an average of all alleles?