BCOOPERMAN.KOLAKOWSKI@BIONET-20.ARPA (04/16/88)
From: Frank Kolakowski <BCOOPERMAN.KOLAKOWSKI@BIONET-20.ARPA> Would someone please post a summary of the effects of the lon protease in E. coli and any relevent experiences with obtaining high levels of expression with and without a defective lon protease. Also, does anyone have a strain which has worked for them. I am interested in expression of yeast pyrophosphatase, and in yeast we have had a time of it with the native yeast proteases. Thanks in advance! Frank Kolakowski jes@eniac.seas.upenn.edu kolakowski%c.chem.upenn.edu@relay.cis.upenn.edu bcooperman.kolakowski@bionet-20.arpa -------