GKOHLHAW@BIONET-20.BIO.NET (Gunter B. Kohlhaw) (01/10/89)
Dear fellow: I am using two plasmids in one yeast cell to study the function of one gene product. What could happen in a yeast cell that contains two plasmids? One of the plasmids contains 2 micron sequence and a TRP1 marker; the other one has a centromere sequence and a URA3 marker. Both plasmids have an ampicilin resistant gene. They are maintained in the cell by mutations of ura3 and trp1 in the genome. I am glad that some body could give me some information about this issue. sciencerely, Kemin Zhou -------