MCFADDEN.UPTON@BIONET-20.ARPA (05/14/88)
From: CHRIS UPTON <MCFADDEN.UPTON@BIONET-20.ARPA>
Does anyone out there have experience using the TAQ DNA polymerase for
DNA sequencing reactions. Our problem is that we are working with large
palindromes and would like to sequence at least one half of the palindrome
of a large number of clones. Previously we have used chemical cleavage or
cut our palindrome in the centre (there is a restriction enzyme that
cleaves at the very centre, AflII, but it is very expensive and not very
efficient) and then used dideoxy- reagents. Both have limited success.
I called Stratagene and the technical dept. told me that various groups had
wanted to use TAQ for sequencing but they didn't have further information
other than the primer had to hybridize at the high temperature and some
changes were necessary in the mixtures
Any information would be appreciated, either of personal experience or of
others I might call.
Chris Upton.
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