GREENGARD.LEWIS@BIONET-20.ARPA (05/15/88)
From: Robert M. Lewis <GREENGARD.LEWIS@BIONET-20.ARPA>
Chris,
I'm not sure this will help with your problem, but there is a fairly
effective method of making progressive deletions in DNA (Henikoff. 1984. Gene
28:351). Modified T7 DNA polymerase (Tabor and Richardson. 1987. PNAS
84:4767) seems to solve a number of problems in sequencing. It is available
as Sequenase from U.S. Biochemical Corp. in Cleveland. They mail out a flyer
with several short technical reports on the use of this enzyme for
sequencing. I am in no way affiliated with U.S. Biochemicals. I hope this
is helpful.
Bob
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