GVYAS.LANE@BIONET-20.BIO.NET (Philip Lane) (12/05/88)
Does anyone know of a commercial supplier of nucleotides or deoxynucleotides (dATP, etc.) which have been chemically modified to add on a fluorochrome moiety (ie. fluoroscein)? I'm especially interested in such fluorochrome-labelled NTPs which also have been modified so that TDT (terminal deoxynucleotidyltransferase) will add only one of them onto the 3-prime end of a single-stranded oligomer. We're doing enough work with the same synthetic oligo probe that I'd really like to get away from 32-p labelling if I can. I know that some suppliers make fluorochrome- labelled PRIMERS (ie. 6-mers) for use in non-radioactive DNA sequencing methods, but what I really need is fluorochrom-labelled single nucleotides. Anyone with any information, I would be forever in your debt! Thanks - Phil Lane bionet: GVYAS.LANE@BIONET; phone 415-476-2750; mail: Transfusion Research Group, Dept. of Laboratory Medicine, Univ. of Calif. San Francisco, Room S-561 Box 0100, 505 Parnassus Avenue, San Francisco, CA. 94143. -------