chend@bionette.ucs.orst.edu (Don Chen - Microbiology) (01/12/89)
Hi: My question involves the best method of coating LPS on ELISA plates. I have extracted LPS from Vibrio (method of Darveau and Hancock). I believe the way to go is to put various dilutions of the antigen in a solution of coating buffer and incubate at 37 degrees C. I would follow with 3% BSA in Tween-TBS, various sera to be tested, mouse monoclonal to the Igs, goat anti-mouse-peroxidase, then developer. Our sera are from various salmonids. Is this correct? Have I most efficiently coated the LPS? (We use Costar EIA/RIA plates). Are there references I should read? What problems should I be aware of? Thanks for any help you can give me. Don Chen Dept of Microbiology Oregon State University Corvallis, OR 97331 (503) 754-3189 email: chend@bionette.ucs.orst.edu