SOKATCH.BURNS@BIONET-20.BIO.NET (P. Gayle Burns) (04/13/89)
Does anyone know of a commercially-available system for purification of
DNA binding proteins? I seem to remember seeing one in which the operator
region of the DNA of interest is cloned into a pUC (or similar) vector.
This recombinant DNA is then attached to an affinity support which consists
of lac repressor protein. This complex is then incubated with crude protein
extract which contains the DNA binding protein. After washing away unbound
proteins,your protein can then be eluted.
If anyone knows of such a product, or is familiar with other techniques
for partially purifying an unknown DNA binding protein, please pass
the information along to me.
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