lah1l@krebs.acc.Virginia.EDU (Lawrence A. Hite) (04/14/89)
I am interested in finding out if anyone has any experience with random priming (vs. oligo dT priming) in the construction of a cDNA library. Promega has a new kit out based on random priming which they claim will make cDNA from non-polyA RNA such as histone RNA (this would not be important in my case as I will be using oligo dT-selected RNA) and they also claim that the average length of clones is larger. Has anyone done side-by side tests of this, and if so what are the advantages/disadvantages with respect to average length, total yield and efficiency of ligagtion of linkers and vector? L A Hite UVa Medical School, Department of Microbiology UUCP: uunet!virginia!lah1l BITNET: lah1l@virginia.BITNET