JLIPSICK@BIONET-20.BIO.NET (Joseph Lipsick) (05/14/89)
I recently saw an ad for a heat-labile bacterial alkaline phosphatase but can't seem to find it again. Does anyone know about this reagent or tried it? It would certainly eliminate a lot of work used to kill conventional BAP or CIP after dephosphorylation. Thanks. Joe Lipsick JLIPSICK -------
lfk@mbio.med.upenn.edu (Lee Kolakowski) (05/15/89)
In article <12493716743.18.JLIPSICK@BIONET-20.BIO.NET> JLIPSICK@BIONET-20.BIO.NET (Joseph Lipsick) writes: I recently saw an ad for a heat-labile bacterial alkaline phosphatase but can't seem to find it again. Does anyone know about this reagent or tried it? It would certainly eliminate a lot of work used to kill conventional BAP or CIP after dephosphorylation. Thanks. I just saw the advertisment: HK(tm) Phosphatase Epicentre Technologies 1202 Ann Street Madison, WI (608) 251-8474 They say something about Antartica ???? maybe an ice bacterium? Also they say: "Is completely and irreversibly inactivated within 30 minutes at 65 C" But what is wrong with CIP anyway, I've not had any problems with heat killing BMB CIP (that I know of). Good Luck. -- Frank Kolakowski ===================================================================== |lfk@mbio.med.upenn.edu || Lee F. Kolakowski | |kolakowski@mscf.med.upenn. || Univ. of Penna. | |c/o jes@eniac.seas.upenn.edu || Dept of Chemistry | |kolakowski@c.chem.upenn.edu || 231 South 34th St. | |bcooperman.kolakowski@bionet-20.arpa || Phila, PA 19104 | |AT&T: 1-215-898-2927 ||--------------------------| |#include <litigate.h> || One-Liner Here! | =====================================================================