JBERNARDIN.HURKMAN@BIONET-20.BIO.NET (Bill Hurkman) (06/02/89)
I was wondering if anyone has used a Nucleogen-DEAE-4000 column (Macherey-Nagel, Duren, F.R.G.) to purify a cDNA insert from the vector after doing a restriction digest. I have spend the last 3 weeks trying to get the method to work and have been unsuccessful. My reference for this work is Hecker, et al [J Chromat. 326(1985):251-261]. If anyone has had success with this column or knows of other references I would appreciate hearing from you. Nina Robinson at jbernardin.hurkman -------