[bionet.molbio.methds-reagnts] PCR * Enzymes cut ss-DNA?

HARTLAUB@dulruu51.bitnet (02/04/90)

Can anybody help me ?

Does anybody know whether larger fragments than the usual 20- to 30-mers
of oligonucleotides can be used as primers for the PCR ?
  (My aim is to connect a certain marker-gene (ca. 1500 bp) instead of a
   restriction-site directly to the PCR-fragments to avoid cloning.)

Which restriction enzymes do cut single-stranded DNA ? (HindIII works).