danielg@uncmed.med.unc.edu (02/01/90)
I have a small technical problem, perhaps one of you can give me a simple
solution.
When I electrophorese my pcr products, I am looking for a band around
200 bases long (pretty small). The problem is that the band usually comes
out right at the dye front, which makes UV visualization a slight problem.
Is there another dye I can use that will run a bit faster or slower than
bromphenol blue? I could try just using glycerol and edta in my loading
buffer, leaving out the dye.
All suggestions welcome...
Daniel G. Sinclair
Rsch Tech II
University of NC, Chapel Hill
Disclaimer: My opinion means | 'If you only knew how much I was holding
nothing, but His | back, you would commend me'
means everything.| - Charles Spurgeon, 19th century
| evangelist (on humor in preaching)tcmaint@tekigm2.MEN.TEK.COM (Thomas A. Dowe) (02/01/90)
I am just a layman, but I was wondering to what degree
reverse-transcriptase(s) are used and the approximate
time they became available for experimental and practical
>uses. Also, to what degree do viral particles remain
in reagent, if any.
Tad
tomd@pulsar.telcom.tek.comaschulman@cc.helsinki.fi (02/13/90)
In article <136@uncmed.med.unc.edu>, danielg@uncmed.med.unc.edu writes: > I have a small technical problem, perhaps one of you can give me a simple > solution. > > When I electrophorese my pcr products, I am looking for a band around > 200 bases long (pretty small). The problem is that the band usually comes > out right at the dye front, which makes UV visualization a slight problem. > > Is there another dye I can use that will run a bit faster or slower than > bromphenol blue? I could try just using glycerol and edta in my loading > buffer, leaving out the dye. > > All suggestions welcome... > > Daniel G. Sinclair > Rsch Tech II > University of NC, Chapel Hill > > Disclaimer: My opinion means | 'If you only knew how much I was holding > nothing, but His | back, you would commend me' > means everything.| - Charles Spurgeon, 19th century > | evangelist (on humor in preaching) You can use xylene cyanol (Sigma X0377 and many other suppliers) which runs more slowly than bromophenol at about .05% final concentration. ASCHULMAN@FINUH.BITNET or ASCHULMAN@CC.HELSINKI.FI