danielg@earl.med.unc.edu (Daniel Gene Sinclair) (02/14/90)
We have just swithed to a non organic dna isolation procedure which is
becoming common... common enough to be out in kit form (Oncor).
The protein is removed in a precipitation step (saturated NaCl) instead
of phenol/chloroform extraction. We ran it along side of a p/c extraction
from whole blood and lymph nodes (chopped), and found yeilds and purity
identical, and found that we had much less problems with restriction of
dna with the non-organic dna's.
No more phenol, hurray! Let me know if you need more info, or call Oncor
at 301-963-3500.
-- dan
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Disclaimer: My opinion means | 'If you only knew how much I was holding
nothing, but His | back, you would commend me'
means everything.| - Charles Spurgeon, 19th century
| evangelist (on humor in preaching)