SCF7@manvax.bitnet ("MYRNA E. WATANABE") (06/01/90)
We are doing atomic absorption spectroscopy of small amounts of desiccated animal tissue (liver and muscle to start) for Pb and Hg content. We are using a Perkin-Elmer AA with a hydride probe. Our sample base is rare and valuable, so we cannot afford to lose it by trying out inappropriate techniques. Our questions are as follows: 1) Does anyone have a reliable method for Hg determination for this type of tissue? Does anyone have any hints or unique techniques? Is there a cold acid digestion method? 2) Can we do the lead determination first and then the Hg (cold technique)? 3) In doing the Pb digestion, we digest first in perchloric acid, then perchloric and nitric acid, heat, then filter with a pump using Whatman #1. Should we be cleaning or rinsing the filter in some way? We appreciate any assistance. Thank you. Please reply to: Myrna E. Watanabe Biology Dept. Manhattan College Bronx, NY 10471 Tel.: 212-920-0266 e-mail: scf7@manvax.bitnet FAX: 914-376-7487