[bionet.molbio.methds-reagnts] non-radioactive DNA detection

J.STERNBERG@aberdeen.ac.uk (07/18/90)

I hope to put a student, who won't have clearance for P32
work for some while, onto doing some restriction mapping
of the ribosomal genes in digenean parasites shortly. So it
seems like we will have to use non-radioactive labelling
techniques on our probes, and I have no experience in
this area. I have a nice glossy booklet from
Boehringer describing their DIG system. It looks OK
but then it should be at the price. I wonder if any
of you have any experience with this or other
non-radioactive labelling methods and any comments
on their sensitivity.
If I get enough responses, I'll collate them and mail
them back to the bboard,
Thanks, Jeremy Sternberg <J.Sternberg@uk.ac.aberdeen>
               <J.Sternberg%uk.ac.aberdeen@ukacrl> (