J.STERNBERG@aberdeen.ac.uk (07/18/90)
I hope to put a student, who won't have clearance for P32 work for some while, onto doing some restriction mapping of the ribosomal genes in digenean parasites shortly. So it seems like we will have to use non-radioactive labelling techniques on our probes, and I have no experience in this area. I have a nice glossy booklet from Boehringer describing their DIG system. It looks OK but then it should be at the price. I wonder if any of you have any experience with this or other non-radioactive labelling methods and any comments on their sensitivity. If I get enough responses, I'll collate them and mail them back to the bboard, Thanks, Jeremy Sternberg <J.Sternberg@uk.ac.aberdeen> <J.Sternberg%uk.ac.aberdeen@ukacrl> (