jes@mbio.med.upenn.edu (Joe Smith) (07/26/90)
We're looking to decrease the time and effort involved in sequence gel
reading. We've looked at two of the 'high-tech' solutions (image
analysis of digitized autorads) and, while the packages are
impressive, they really don't seem to solve the problem. Further,
cost (20-30K$) makes them very difficult to justify.
The next step down is some combination of human and computer, either a
hand-held pen digitizer or a voice recognition system. These are
within our budget and we are trying to get demos in the lab to try
out. In the meantime, if you are using one of these (or want to
suggest an alternative I haven't considered!) we would be very
interested in your experience and suggestions.
>Joe
--
Joe Smith
University of Pennsylvania jes@mbio.med.upenn.edu
Dept. of Biochemistry and Biophysics (215) 898-8348
Philadelphia, PA 19104-6059arenas@citiago.bitnet (Jaime Arenas) (07/28/90)
> The next step down is some combination of human and computer, either a > hand-held pen digitizer or a voice recognition system. These are > within our budget and we are trying to get demos in the lab to try > out. In the meantime, if you are using one of these (or want to > suggest an alternative I haven't considered!) we would be very > interested in your experience and suggestions. Hi there.. I am very happy with the way I read my sequences and I would not change it for any other method that I know. Is as follows: A computer terminal is set on a table which has a built-in light box. The table and light box area are large so the terminal can be to my left, the keyboard to my right and the iluminated autoradiogram in front of me. So I put my right hand in the keyboard in such a way that each one of the A,G,C and T are under one of my fingers. This way I dont need to look at the keyboard or the monitor any more. Then, using my right hand to guide myself through the sequence it flows (the sequence) directly to the computer as fast as I can read it. When I started to do this I had my doubts about the rate of mistakes. However, I found that mistakes were less than when somebody else is reading an you are typing. The effort...... it only takes what it takes to read it!! The cost of it is what it takes to get a good carpenter to make your own design of the "reading bench" assuming that you already have acomputer. Of course The Human Genome Proyect may want to use other methods..... Jaime Arenas Ph.D. There is no places, Division of Biology 147-75 there is beginnings and ends, CALTECH but nothing is ever forgotten. Pasadena, CA 91125 ARENAS@CITIAGO.BITNET ARENAS@IAGO.CALTECH.EDU (NO, there are no misspellings)