FRIEDMAN@BIOVAX.RUTGERS.EDU (08/14/90)
I have been trying for a while to clone several random fragments that I obtained from D. melanogaster into a 16kb vector. The fragments (6-12+kb) are already cloned into pUC19 vectors at the site (KpnI) which I want to use to clone into the 16 kb vector. I have phosphotased the vector and it seems to not self ligate. The colonies I do get do not appear to be the product of the ligation. Any suggestions as to concentrations buffers other than standard (Maniatis) would be helpful Rich Friedman@MBCL.rutgers.edu Friedman@biovax.rutgers.edu