Jim Gerlach <GERLACHJ@QUCDN.QueensU.CA> (09/05/90)
We are doing some southern blots of PCR'd cDNA and have been separating a 650bp DNA fragment using a gel consisting of 2.75% wide-range agarose (Sigma) and 1.0% standard agarose (Pharmacia). We seem to be getting poor capillary transfers to nitrocellulose and wonder if this mix may be contibuting. Has anyone had experience with transfers from this or similar agarose mixes? Thanx in advance for any suggestions, comments, etc. Jim ------- James.H.Gerlach@QueensU.CA Queen's University GERLACHJ@QUCDN.QueensU.CA (NetNorth/BITNET) Room 358, Botterell Hall tel (613) 545-6446 Kingston, Ontario fax (613) 544-9708 CANADA K7L 3N6