steffen@raven.bcm.tmc.edu (David Steffen) (10/11/90)
Some weeks ago, I asked for any information about inhibitors of o-linked glycosylation. I received 3 replies. (Because the replies were by email, I will not quote the authors names). Interestingly, they provided rather different answer! Responder#1 said "Apart from a serine or theonine analogue that also blocks translation, no o-lined glycosylation inhibitors exist" Responder#2 said "MONENSIN...will interfere with o-linked glycosylases. I used this drug a number of years ago when characterizing the surface glycoproteins of human Paramyxovirus III. Monensin is an ionophore that interferes with many cellular functions: Bennet et al. Cell Tissue research 250:355 Cheng et al. 1987 Endocr. Res. 13:243 Johnson and Spear 1982 J. Virol. 43:1102 Kousoulas et al. 1983 Intervirology 20:56 Machamer and Cresswell 1984 PNAS 81:1287 Tartakoff 1983 Cell 32:1026 Sources: Sigma, Calbiochem" Responder#3 said referred us to JBC v264 #32 pp19271-19277 (1989) for a list of other inhibitors. This looks like a case where some posting directly to this group might be enlightening to us all! I originally posted this request for a graduate student, Jot Singh, who is now "on line" and will participate in any resulting discussion himself. If I can twist his arm, he will follow up in some weeks with his own results. -David Steffen-