steffen@merlin.mbir.bcm.tmc.edu (David Steffen) (11/27/90)
In article <1990Nov26.233931.1@csc.fi> pollanen@csc.fi writes: >What problems have you had [doing PCR directly from cells]? We have had good luck doing PCR from animal cells (Rat1-based lines, I believe) with no particular problems. Our protocol was not particularly unique, and thus I hesitate to post it, but send me mail if you would like me to email it to you. >I have tried to do PCR from so called VIRAPAP samples [w/o luck] I have no idea what VIRAPAP is. We always started with growing cells. -- David Steffen Department of Cell Biology, Baylor College of Medicine, Houston TX 77030 Telephone = (713) 798-6655, FAX = (713) 790-0545 Internet = steffen@mbir.bcm.tmc.edu
pollanen@csc.fi (11/27/90)
Hello Has anyone done PCR directly from cell samples and what problems have you had if you had done PCR directly from cell samples... I have tried to do PCR from so called VIRAPAP samples but I have not succeeded well can you help me yours Raimo Pollanen (M.Sc.) E-mail POllanen@csc.fi