USERBFFO%SFU@PUCC.PRINCETON.EDU (Francis Ouellette) (11/20/90)
In a recent message Johan de Boer (FS300512@YUSOL) asks: >I am trying to find a source for the following restriction >enzyme: > > SplI or PfuI or PspI > >They are isoschizomers. If anyone knows of a company that >sells one of these, would you please let me know? If I look up in PC/Gene using the REDIT program (the restriction enzyme editor) I find the following info: > >RESTRICTION ENZYME DATA EDITOR. > >Enzyme name >Recognition site >Cut offset: 1__ bases. > >Isoschizomers. > >Availability. > > The (old) Amersham catalogue I have (1989) claims that Amersham is the only distributor of Spl I. But PC/Gene doesn't seem to know that Spl I has any isoschizomers ... But the REDIT program of PC/Gene is a bit out of date ... Maybe the next update ;-) disclaimer: I am in no way, shape or form an employee or a sales rep for IntelliGenetics or Amersham. "And that's the truth Ruth" (Do the right thing, Spike Lee) bffobffobffobffobffobffobffobffobffobffobffobffobffobffobffo "Je cherche a comprendre" Jacques Monod B.F. Francis Ouellette Bitnet (Netnorth): userBFFO@SFU Internet: userBFFO@cc.sfu.ca Ouellett@whistler.sfu.ca address: Dept of Biol. Sci., Simon Fraser University Burnaby, BC, V5A 1S6, Canada, (604)291-3021 FAX: (604)291-3496 bffobffobffobffobffobffobffobffobffobffobffobffobffobffobffo PS. I realize that being from Canada it may be politically unwise (and this be the wrong place for it) to have a signature "en francais" but hey, pourquoi pas! ;-)
jes@mbio.med.upenn.edu (Joe Smith) (11/28/90)
> The (old) Amersham catalogue I have (1989) claims that > Amersham is the only distributor of Spl I. The latest IG RE lists (compiled from R. Robert's 9009 list) agree (I don't know how recent the manufacturer's lists are). The list doesn't have PspI. renz: searching restfile.lst for '\<spli\>'... (list any isoschisomers) ---- BsiWI (SplI) CGTACG Pfl23II (SplI) CGTACG PfuI (SplI) CGTACG PpuAI (SplI) C^GTACG SmaAI (SplI) CGTACG SplI C^GTACG SplAI (SplI) CGTACG SspKI (SplI) CGTACG (check for sources...) renz: nothing matches 'BsiWI' renz: nothing matches 'Pfl23II' renz: nothing matches 'PfuI' renz: nothing matches 'PpuAI' renz: nothing matches 'SmaAI' amersham:SplI C^GTACG (nobody but Amersham, takara:SplI C^GTACG (and Takara renz: nothing matches 'SplAI' renz: nothing matches 'SspKI' -- Joe Smith University of Pennsylvania jes@mbio.med.upenn.edu Dept. of Biochemistry and Biophysics (215) 898-8348 Philadelphia, PA 19104-6059