DBK@aberystwyth.ac.uk (12/03/90)
Does anyone know a good enzyme (and source!) for degrading yeast (Saccharomyces cerevisiae) cell walls to make protoplasts? We have had a go with Sigma lyticase (mixture of stuff from Arthrobacter), which certainly lives up to its name (i.e. clobbers the protoplasts after making them). Once upon a time there was helkicase from snails, which is supposed to be good (not in Sigma catalogue). [that was helicase!] All help *VERY* gratefully received. Many thanks, Douglas Kell.
arenas@citiago.bitnet (Jaime Arenas) (12/07/90)
> Does anyone know a good enzyme (and source!) for degrading yeast > (Saccharomyces cerevisiae) cell walls to make protoplasts? We have > had a go with Sigma lyticase (mixture of stuff from Arthrobacter), which > certainly lives up to its name (i.e. clobbers the protoplasts after making > them). Once upon a time there was helkicase from snails, which is supposed to > be good (not in Sigma catalogue). [that was helicase!] We always use Zymolyase from SEIKAGAKU with good results. ZYMOLYASE - 100T CAT# 120493 SEIKAGAKU AMERICA INC. P.O. BOX 21517 St. Petersburg, FL 33742 info (800) 237-4512 telex 52-9422 SKK STB Fax (813) 576-1870 --------------------------------------------------------------------------- Jaime Arenas, Ph.D. Division of Biology 147-75 CALTECH Pasadena, CA 91125 ARENAS@CITIAGO.BITNET ARENAS@IAGO.CALTECH.EDU