D54%TRANAVM1@PUCC.PRINCETON.EDU (suleyman AYDIN) (12/06/90)
Here in the microbiology lab. of our medical fac., we have problems
with Agrobacterium tumefaciens. Our friends could not be able to passage and
have live forms continuously. They said that this is natural since they do
not have any single information about this bacterium.
These bacteria arrived our research centre from U.S. and i do not have
enough info about them..so i can not give info to the microbiology lab. staff.
1.What must the groth medium consist (agar? blood.... etc?)
2.What must be the growth temperature (shall we leave them in room temp. after
passage..?)
3.Does it need a special moisture percent?
4.Can we have it for long years just by passaging it (in suitable conditions,
if we can learn them..)
5.How many times can it be passaged normally? Is there any limitations?
6.What must be the frequency of passages? (e.g., one month?)
7. Other useful knowledge is also appreciated (especially by the microbiology
lab stuff)..:-)
If anyone has information about this bacterium, please help otherwise we
shall loose all of them.
Thanks a lot.
suleyman AYDIN (Biologist)
medicinal plants res. ctr., univ. anatolia, tepebasi - eskisehir 26470 /Turkey
e-mail:d54@tranavm1.Bitnet
...... suleyman the gateway to campus......LAFLAMME@MAINE.BITNET (12/11/90)
I have worked with A. tumefaciens and I have found them to be fairly easy to handle. Most of the ones I have used are common plant transformation strains so I dont have any special information for you if you are using wild strains. Most of these grow at 28 degrees or so in simple media such as LB. They do grow fairly slowly and tend to clump together so dont expect a nice culture like you might get with E coli. Some of these strains carry antibiotic resistance genes on the Ti plasmids so it might be wise to include it if loss of the plasmid is of concern to you (but i suspect that it would be difficult to lose such a large plasmid). If you have any other questions please feel free to contact me directly, if I cant help you I can probably point you in the right direction. ********************************************************************* * * * * Dan Laflamme * * * Dept. of Biochemistry * * * University of Maine * * * Orono, Maine 04469 * * * (207) 582-2818 * * * * * *********************************************************************