launce@apollo.med.utah.edu (Launce Gouw) (12/13/90)
A little while ago discussion came up regarding Invitrogen's new TA cloning system which claims to be able to copy unmodified (i.e., no special primers needed) PCR products into their pCR2000 vector by utilizing the ragged ends generated by the terminal transferase activity of thermophilic polymerases. (How's that for a run-on sentence :-) Anyway, has anybody tried it yet? What d'ya think? After calling them, they said they don't give out trial samples. We're not quite ready to shell out the ~ $240/20 reactions they're charging, especially since we don't have enough information (anecdotal or otherwise) on the product, other than their advert blurbs and brief info flier. If anybody who's used the product or knows somebody who's used the product could reply to me (by email or by posting) I'll summarize for others who might be interested about results + feedback regarding this new tool. ADVthanksANCE -- ___ / / _____ / /___/ ___/ "What a silly bunt!" /_____ /_---- /____/ launce@apollo.med.utah.edu *** help! help! i'm being repressed!! **we apologise for the previous opinions. those responsible have been sacked**