friedman@mbcl.rutgers.edu (01/16/91)
My original posting >We recently received a plasmid for which we have no information. The plasmid, >we are interested in studying. However, due to the lack of sequence info or a >restriction map of this plasmid, we are kind of stuck. There is no mention of >this in any databank we have available. Does anybody know anything about this >plasmid? >Please e-mail to >Friedman@mbcl.rutgers.edu >Thanks >Rich Friedman I was didn't notice that after the pasting of the text into the news group, the plasmid name was left off. The plasmid is pW8. It is apparently a P-element vector used for inserting DNA into the genome of Drosophila melanogaster.
gilbertd@cricket.bio.indiana.edu (Don Gilbert) (01/16/91)
In article <247.27934eb5@mbcl.rutgers.edu> friedman@mbcl.rutgers.edu writes: > >I was didn't notice that after the pasting of the text into the news group, the >plasmid name was left off. The plasmid is pW8. It is apparently a P-element >vector used for inserting DNA into the genome of Drosophila melanogaster. If this is any help, there is a PW8(something) mentioned in John Merriam's Drosophila clone list. Here are the entries. clonelist.txt:7D1,2 [PW8hsp-sev ch41]hsp,sev 3 0 Science243:931 clonelist.txt:46E [PW8hsp-sev ch21]hsp,sev 3 0 Science243:931 clonelist.txt:93B [PW8hsp-sev ch50]hsp,sev 3 0 Science243:931 The first column is cytological map location, the next [PW8...] is gene name the third ("3") is "transformed inserts", the fourth column ("0") is size in kilobases, and the last is a reference. This clonelist is available via anonymous ftp from iubio.bio.indiana.edu, directory [archive.fly] -- Don -- Don Gilbert gilbertd@cricket.bio.indiana.edu biocomputing office, biology dept., indiana univ., bloomington, in 47405
fischgf@milton.u.washington.edu (Guadalupe Fisch) (01/17/91)
Does anybody know the name and source of a good mitecide (for killing mites on a drosophila stocks.) Please send info to fischgf@milton.u.washington.edu Thanks, Lupe