bwoodman@magnus.ircc.ohio-state.edu (Robert H Woodman) (02/16/91)
Hello, fellow bionetters! I have for a considerable period of time desired to have a particular kind of plasmid for use with the genus _Streptomyces_. The plasmid I have in mind is an _E. coli_/_Streptomyces_ shuttle vector. The plasmid should *not* contain the resistance gene for thiostrepton/nosiheptide. The resistance gene I wish to see on the plasmid is a penicillin/ampicillin resistance (beta-lactamase) gene. The gene should either be expressible in both _Streptomyces_ and _E. coli_ (as you may know, _E. coli_ can use some _Streptomyces_ promotors), or there should be two copies of the gene -- one each for expression in the two different organisms. Finally, the plasmid should have a useful polylinker or at least several useful cloning sites. If anybody has such a plasmid (or even just a beta-lactamase expressible in _Streptomyces_), and if you can share it, would you please let me know? I'd like to work with such a plasmid in _Streptomyces actuosus_, the nosiheptide producer. (Note: I'm not too lazy to invent such a plasmid if one doesn't exist, but I thought that before I tackled such a project, I'd make sure that I wasn't re-inventing the wheel.) Thanks for any help offered. Robert H. Woodman -- ********************************************************************* *Bob Woodman * "A job not worth doing well is not * *INTERNET: woodman.1@osu.edu * worth doing."--Salvador Luria * *********************************************************************