wyk238@csd4.csd.uwm.edu (Wen-Yen Kao) (03/04/91)
Sorry if I missed the earlier part of the glassmilk discussions. Here is the question. This is what I heard from a BIO 101 salesrep at last year's ASCB San Diego meeting. He explained to me that the reason why glassmilk does not bind to small DNA fragment (say, < 500 bp) too well is because of the pH. When we need to isolate the smaller fragment, all we need is to lower the pH (it may be what they do for the Mermaid). Since I haven't been doing any of fragment isolation lately to see how that works out, I was wondering if any bionetter has a home recipe for this, too? -wenyen (wyk238@csd4.csd.uwm.edu)