cthomas@f.adelaide.edu.au (03/04/91)
Hi, Does anyone have a reliable protocol for extraction of total RNA/mRNA from Gram positive bacteria like Bacillus and Listeria. Most of the the published methods I have seen apply to easily lysable Gram negative bacteria or eucaryotic cells. Thanks in advance, Connor Thomas Department of Microbiology and Immunology University of Adelaide South Australia Email: cthomas@f.adelaide.edu.au ------------------------------------------------------------------------------
kim@m44.unm.edu (03/05/91)
In article <1991Mar4.135643.1@f.adelaide.edu.au>, cthomas@f.adelaide.edu.au writes: >Hi, >Does anyone have a reliable protocol for extraction of total RNA/mRNA from >Gram positive bacteria like Bacillus and Listeria. Most of the the >published methods I have seen apply to easily lysable Gram negative bacteria >or eucaryotic cells. > >Thanks in advance, > >Connor Thomas >Department of Microbiology and Immunology >University of Adelaide >South Australia >Email: cthomas@f.adelaide.edu.au > >------------------------------------------------------------------------------ In Analytical Biochemistry 176:464-467 [1989], there is a procedure for preparing bacterial DNA using DMSO to lyse the cells. It may be of use in your application. I have never used the method myself. Daniel Kim