EBMV36@ercvax.edinburgh.ac.uk (03/11/91)
Is there anyone who has the experience of doing genomic southern hybridization using vertical electrophoresis? According to the adv. of Stratagene, they claimed the vertical electrophoresis gives better resolution and saves the running time. I am wondering why there is just only one model of commercial electrophoresis apparatus available if the vertical agarose electrophoresis has such wonderful magics. Any comment and discussion would be appreciated. Chao-Liang Wu Dept. of Molecular Biology Univ. of Edinburgh EBMV36@ercvax.edinburgh.ac.uk
wrp@biochsn.acc.Virginia.EDU (William R. Pearson) (03/12/91)
In article <9103100855.AA04653@genbank.bio.net> EBMV36@ercvax.edinburgh.ac.uk writes: >Is there anyone who has the experience of doing genomic southern hybridization >using vertical electrophoresis? According to the adv. of Stratagene, they >claimed the vertical electrophoresis gives better resolution and saves the >running time. I am wondering why there is just only one model of commercial >electrophoresis apparatus available if the vertical agarose electrophoresis >has such wonderful magics. >Any comment and discussion would be appreciated. > >Chao-Liang Wu >Dept. of Molecular Biology >Univ. of Edinburgh >EBMV36@ercvax.edinburgh.ac.uk I am a great fan of vertical gel electrophoresis for Northern and Southern blots. I believe that it provides a quality and reproducibility that is difficult to match with horizontal apparatus. We use gel boxes made be Watson Products, PO Box 247, (818) 797-5773. They are a "mom & pop" company that started making vertical gel apparatus just as agarose was being developed for DNA fractionation. They sell frosted (sand-blasted) plates, which are essential for holding low-percentage gels in position. Bill Pearson