bia60%uk.ac.keele.seq1@PUCC.PRINCETON.EDU (N.G. Morgan) (03/21/91)
I have recently received details of a chemiluminescent substrate for alkaline phosphatase (AMPDD - Boehringer) which can be used to visualize nucleic acids on Northern & Southern blots. Boehringer tell me that they are unaware of any reports of its use to localize protein bands on Western blots after incubation with antibody conjugated to alkaline phosphatase. I should like to hear from anyone who has tried this - are there particular problems that preclude its use on protein blots? Thanks, Noel Morgan. Dept. of Biological Sciences, University of Keele, Staffs U.K. Email: bia60@uk.ac.keele.seq1