PHILLIPSA@lars.afrc.ac.uk (04/12/91)
A colleague of mine is using GUS as a reporter gene for promoter action in a parasitic fungus, and using X-GLUC for histochemical assay. He doesn't seem to be getting very good signal strength, despite using a "very strong" promoter. Does anyone have any tips for optimizing GUS/X-GLUC histochemistry, especially in fungal hyphae? Andy Phillips (for John Keon) Long Ashton Research Station, Birstol, UK