pollanen@csc.fi (04/26/91)
HELLO HAS ANYONE EXPERIENCE WITH PROMEGA's PGEM -3Z AND PGEM -4Z CLONING VECTORS.... I HAVE TRIED TO LIGATE ABOUT 3.7-3.9Kb INSERT INTO THEM BUT NOT SUCCEEDED WHAT THINGS DO I HAVE TO CHECK WHICH PROBABLY INHIBIT T4 DNA LIGASE ENZYME? YOURS RAIMO POLLANEN(M.S.)