jjw@rsbs0.anu.edu.au (04/29/91)
I have a question regarding the different sources of Biotin derivatized nucleotides. I'm intending to set up a Biotin/Alk-Phos/Dioxetane non-isotopic detection system and I'm quite staggered at the range of prices from different suppliers of Biotinylated nucleotide bases. For eg. Beohringer-Mannheim quotes Au$156 for 50 nmoles of Biotin-16-dUTP and Sigma quote US$104 (~ Au$130) for 0.5 mg of Biotin-11-dUTP !!! Both are supplied at 85% purity. Has anybody out there tried different suppliers? With what success? Any lessons learnt which you could pass on? If you reply back to me I'll summarize to the net. Thanking you all in advance, ******************************************************************************** Jeremy Weinman Plant-Microbe Interaction group Research School of Biological Sciences Australian National University Email: jjw@rsbs0.anu.edu.au Phone: 61 6 2495051 Fax: 61 6 2490754 Snail: PO Box 475, Canberra, ACT 2601, AUSTRALIA ********************************************************************************