[bionet.molbio.methds-reagnts] westerns

SCOLNIPA%ESVAX@dupont.com (05/02/91)

Is it possible to quantify western blots? Also, I'd rather not use I125.
I have a good polyclonal antibody and purified protein.  Can 
chemoluminescence be used in a quantitative fashion?  Thank you in advance 
for any help on this matter.

PC120@biology.cambridge.ac.uk (05/02/91)

I have with good reproducibility used normal densitometry on top of a
illumination board for quantification of Western blots.

Peter Carlsson, PhD
Dept. of Biochemistry
Univ. of Cambridge

PC120@UK.AC.CAMBRIDGE.BIOLOGY