wrp@biochsn.acc.Virginia.EDU (William R. Pearson) (05/29/91)
Savant, the people who make Speed-Vacs, have introduced a pump designed for gel-drying and similar problems, where a large amount of potentially corrosive liquid is removed. It is a diaphram pump with no oil. Unfortunately, it costs around $1500. Looks like it would do the job. Bill Pearson
PHILLIPSA@lars.afrc.ac.uk (05/29/91)
I've never been in a laboratory which has a wholly satisfactory system for drying polyacrylamide and agarose gels. The BioRad gel dryers are fine, it's the vacuum pumps which present the problem. Most labs I've been in use in-line vapour traps to remove the corrosive solvents, but these don't seem to be very efficient and the vacuum pump has a limited life even with frequent oil changes. Our present system uses an Aquavac, which (as I understand it) is a water vacuum pump driven by high-pressure water. This is OK, but doesn't give the greatest vacuum and ours needs replacing anyway. To get to the point, I've come across literature for a pump made by Genevac, which uses a Cole pump system. This seems ideal (although not cheap), as it has few moving parts and is highly corrosion-resistant. Has anyone experience of this type of pump with corrosive vapours? Can someone recommend a better system? Thanks for your help Andy Phillips Long Ashton Research Station, Bristol, UK JANET: PHILLIPS@UK.AC.AFRC.LARS EARN/BITNET: PHILLIPSA@LARS.AFRC.AC.UK
donnel@helix.nih.gov (Donald A. Lehn) (05/30/91)
In article <9105291140.AA01516@genbank.bio.net> PHILLIPSA@lars.afrc.ac.uk writes:
->I've never been in a laboratory which has a wholly satisfactory
->system for drying polyacrylamide and agarose gels. The BioRad gel
->dryers are fine, it's the vacuum pumps which present the problem.
->Most labs I've been in use in-line vapour traps to remove the corrosive
->solvents, but these don't seem to be very efficient and the vacuum pump
->has a limited life even with frequent oil changes. Our present system
->uses an Aquavac, which (as I understand it) is a water vacuum pump driven
->by high-pressure water. This is OK, but doesn't give the greatest vacuum
->and ours needs replacing anyway.
->To get to the point, I've come across literature for a pump made by
->Genevac, which uses a Cole pump system. This seems ideal (although not
->cheap), as it has few moving parts and is highly corrosion-resistant.
->Has anyone experience of this type of pump with corrosive vapours?
->Can someone recommend a better system?
->
->Thanks for your help
->
->Andy Phillips
->Long Ashton Research Station, Bristol, UK
->JANET: PHILLIPS@UK.AC.AFRC.LARS
->EARN/BITNET: PHILLIPSA@LARS.AFRC.AC.UK
Regarding your input for information on the Genevac. We have a Genevac pump
model number CVP100/2 in our lab here at the National Institutes of Health.
Based on how well this pump works and the type of service we have had from the
company I feel you would have to be a complete fool to believe that this
product actually works. In the 18 months that we have had the pump it has
actually worked for a total of not more than 116 hours. This is not because
we do not use it but because the pump has broken down virtually every time we
have tried to use it.
The service from Genevac is even less efficient than their pump. The pump
originally stopped working several hours after the company service rep came
to the lab and set it up. It took more than 6 weeks before we could get the
pump serviced. After the pump was serviced, a small hose broke on the pump
broke and it took more than 2 months in order to get a replacement. Currently,
the pump is once again down. We called Genevac several months ago in order to
have it serviced and we are still waiting for the repair man.
Based on my experience with Genevac and their poor service record, I would
highly recommend against dealing with this company. If you need a reliable
vacuum system, buy a traditional high quality vacuum pump and put on it the
filter system sold by Svant. I have found that the Savant filter system is
excellent in protecting the pump connected to a gel drier.
Best regards,
Donald A. Lehn
National Institutes of Health
Email: donnel@helix.nih.govBROE@AARDVARK.UCS.UOKNOR.EDU (Bruce Roe) (06/01/91)
Fellow Netters, With this discussion of refrigerated traps and pumps for gel drying, thought I'd put in my 2-cents worth. We have two FTS-system Multi-Cool Refrigerated traps on two separate gel dryers and they satisfactorly protect the pumps *if* we perodically change the oil. /\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\ \ Bruce A. Roe INTERNET: BROE@aardvark.ucs.uoknor.edu / / Dept. of Chem. and Biochem. BITNET: BROE@uokucsvx \ \ University of Oklahoma AT&TNET: 405-325-4912 or 405-325-7610 / / 620 Parrington Oval, Rm 208 FAXnet: 405-325-6111 \ \ Norman, Oklahoma 73019 ICBMnet: 35deg 14min N, 97deg 27min W \ \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/ /o o\ \ /\ /\ /\ /\ /\ /\ /\ /\ /\ /\ /\ /\ /\ /\ /\ /\ /\ /\ /\ /\ /\ /\ o X X X X X X X X X X X X X X X X X X X X X X \O/ / \/ \/ \/ \/ \/ \/ \/ \/