vivien@ariel.ucs.unimelb.edu.au (Vivien Bonazzi) (06/05/91)
I wanted to make a few inquires about PCR machines that are available on the
market at the moment. I'm thinking of buying a machine for our lab.
If anyone has experience/information about the following machines could you
pass it on to me. Any other information or suggestions about which machines
are better would also be appreciated..
Thanks
Vivien
Thermocycler TPS 20 or TPS 5*4
Perkin Elmer 480
Savant Temperature Cycler TC-49
PREM Series III thermocycler
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Vivien Bonazzi email vivien@ariel.ucs.unimelb.EDU.AU
Dept. Pharmacology
University of Melbourne
Australia
*******************************************************************************harper@csc.fi (06/05/91)
In article <750@ariel.ucs.unimelb.edu.au>, vivien@ariel.ucs.unimelb.edu.au (Vivien Bonazzi) writes: > > > I wanted to make a few inquires about PCR machines that are available on the > market at the moment. I'm thinking of buying a machine for our lab. There has been quite alot written about PCR in the METHODS Bboard. The entries go back to 1990. There is about 90 hits using LDBASE and SEARCH PCR IN +METHODS. Here are the results. You can recover the items you want to read about from LISTSERV by sending a small batch file to LISTSERV@IRLEARN.UCD.IE ( I would just like to say that the technique of using LDBASE or MAIL BATCH processing is VERY SIMPLE and fast. I got the results from LDBASE instantaneously and the batch mail was delivered within 15 minutes. The facilities are available use them.) Here is an example of a batch file to get items dealing with PCR/MACHINES Just cut it out and post it to LISTSERV@IRLEARN.UCD.IE Leave the subject line blank and in the main body of the mail include the following: ----------------- 8< cut here 8<---------------- //methods JOB Echo=YES,Reply-via=MAIL Database search DD=Mine //Mine DD * Search pcr in +methods index print 000260 print 000279 print 000266 print 000470 print 000481 print 000485 /* ---------------- 8< cut here 8<----------------- I enclose the index of items so that if you wnat to ADD more PRINT commands to the above batch job you can do so... perhaps there are other things that you might be interested in. ************************* CLIP ********************* > INDEX Item # Date Time Recs Subject ------ ---- ---- ---- ------- 000012 90/01/26 07:53 20 PCR....help 000013 90/01/26 18:02 40 RE: PCR....help 000014 90/01/31 17:13 31 visualizing small pcr fragments 000015 90/02/01 10:54 18 Re: visualizing small pcr fragments 000016 90/02/01 09:33 36 Small PCR fragments. 000018 90/01/29 16:20 39 Re: PCR....help 000019 90/02/03 19:25 18 PCR * Enzymes cut ss-DNA? 000020 90/02/12 17:55 40 Re: visualizing small pcr fragments 000028 90/02/12 18:14 26 Re: visualizing small pcr fragments 000030 90/02/20 21:48 36 UV light kills DNA contamination in PCR samples 000032 90/02/21 11:22 12 Primers for PCR 000034 90/02/23 20:11 39 re: PCR...help 000061 90/04/11 11:25 21 PCR ON YEAST RNA 000064 90/04/19 23:32 29 Computer program for choosing oligonucleotides (qu+ 000065 90/04/29 23:33 23 Looking for cheap Thermal Cyclers 000080 90/05/29 18:12 17 000085 90/05/30 13:55 65 Re: (none) 000099 90/06/19 10:25 17 PCR errors 000102 90/06/25 06:40 32 Re: PCR errors 000123 90/07/11 17:28 46 000124 90/07/11 14:55 21 LONG PCR FRAGMENTS 000134 90/07/21 08:18 16 PCR reagent software 000146 90/07/27 13:37 20 pcr primer dimer help 000151 90/07/31 16:41 33 Bacteria and PCR 000184 90/09/05 00:39 24 Southerns with seiving agarose 000186 90/09/06 14:24 20 melting algorithms 000191 90/09/12 14:39 48 Cloning PCR fragents into vector 000192 90/09/13 10:00 75 Re: Cloning PCR fragents into vector 000193 90/09/14 01:53 70 Re: Returned Network Mail 000197 90/09/18 12:14 25 melting algorithms 000203 90/09/27 02:27 81 Re: Oligo directed Insertions 000206 90/09/28 13:29 51 PCR Sequencing 000208 90/10/01 12:08 17 re:pcr sequencing 000210 90/10/02 09:38 60 RE: PCR sequencing 000213 90/10/02 12:51 25 re:PCR sequencing 000220 90/10/09 12:15 35 DNA ISOLATION 000229 90/10/04 17:16 28 VNTR sequencing 000242 90/10/23 13:53 21 000243 90/10/23 17:59 24 asymmetric PCR + sequencing 000244 90/10/24 19:36 26 RNA to cDNA then PCR 000248 90/10/29 21:30 23 Re: Oligonucleotide probing 000250 90/11/02 06:31 26 PCR Primer Selection Programs Required 000251 90/11/02 14:53 27 alu seqs and pcr 000252 90/11/02 15:54 19 PCR cloning 000254 90/10/28 12:41 38 Re: inverse PCR 000255 90/11/02 14:54 27 alu seqs and pcr 000256 90/11/05 09:27 16 Re: Oligonucleotide probing 000257 90/11/05 11:50 22 PCR CLONING 000258 90/11/05 17:54 21 re:PCR CLONING 000259 90/11/05 17:03 20 000260 90/11/08 13:49 29 PCR Machine 000266 90/11/10 01:58 45 Re: PCR Machine 000279 90/11/15 17:32 26 Re: PCR Machine 000288 90/11/26 23:39 19 about PCR problems 000289 90/11/26 23:15 25 Re: about PCR problems 000300 90/12/12 20:54 35 PCR Cloning - TA Cloning system 000302 90/12/13 14:04 33 PCR cloning 000314 91/01/08 23:20 24 Anyone done PCR directly from cells 000315 91/01/08 23:04 24 Re: Anyone done PCR directly from cells 000316 91/01/10 07:36 15 Re: Anyone done PCR directly from cells 000319 91/01/12 11:01 20 Re: Anyone done PCR directly from cells 000320 91/01/15 07:18 18 Re: Anyone done PCR directly from cells 000336 91/01/28 19:17 21 bacteria detection in plants 000338 91/01/31 04:44 184 seeking bacterial strains 000339 91/01/31 16:11 28 Direct PCR from Tissue Samples 000351 91/02/04 19:38 20 pcr on fixed samples 000359 91/02/18 16:14 57 message 000366 91/02/20 22:54 135 message 000391 91/03/06 13:41 16 PCR 000401 91/03/11 00:54 21 Help: DNA from Clostridium spores 000404 91/03/12 15:53 30 PCR fragment cloning into m13 vectors 000406 91/03/12 15:57 61 Re: PCR frag. cloning into M13 vectors 000407 91/03/12 13:39 33 Re: PCR fragment cloning into M13 vectors 000416 91/03/15 17:30 47 Re: PCR fragment cloning into m13 vectors 000418 91/03/15 17:58 50 Re: PCR fragment cloning into m13 vectors 000433 91/03/30 21:34 74 Why does RAPD mapping work? 000439 91/04/06 02:55 116 Re: Why does RAPD mapping work? 000449 91/04/12 17:24 352 Re: Why does RAPD mapping work? SUMMARY 000456 91/04/23 18:15 40 PCR primer sequence collection 000459 91/04/26 18:30 22 Genomic substraction 000461 91/04/29 09:49 36 Reverse Transcription and PCR 000464 91/04/29 19:40 99 Re: Genomic substraction (fwd) 000470 91/05/03 18:37 20 Unreliability of PCR machines in time 000471 91/05/03 20:32 12 PCR 000472 91/05/06 16:59 28 000481 91/05/14 17:27 14 pcr MACHINE 000482 91/05/15 09:38 21 Facilities-Primers Design? 000483 91/05/14 19:31 44 PCR Machine-MJ Research 000485 91/05/15 10:16 16 RE: pcr MACHINE 000506 91/05/29 18:20 170 summary of *oligo construction programs* 000508 91/05/30 16:29 23 PCR references? ****************** END ************************ Rob "I'd rather see a sermon than hear one" Harper P.S. This months special offer: I will do LDBASE searches for anyone who wants to recover old messages from LISTSERV and provide them with an INDEX of entries... but they with have to finish the job off themselves and do the actual recovery by BATCH MAIL. Looking forward to hearing from you.