[bionet.molbio.methds-reagnts] Analysis of plasmid after transfection?

klautky@vaxa.weeg.uiowa.edu (BIOCHEMISTRY) (06/06/91)

Dear Net Readers,

    Does anyone know of a protocol (or direct me to a reference) for the
analysis of plasmid DNA after transfection?

    I am interested in a method(s) that can quantitate the plasmid transfected
and determine intactness with respect to supercoiling.  Obviously, a
Southern can be used to check the latter but it is the former that I am
unsure of.

                Thanks in advance,                 Stephen Klautky