ALSOBROOK@yalemed.bitnet (06/08/91)
Well, if you're going to do a Southern, one way to quantitate the plasmid is to use known quantities of the plasmid on the same blot. After hybrid- ization, you can use the known plasmid signals as a calibration curve. Even without a densitometer, this will give a fairly accurate estimate of how many plasmid copies are present.