pollanen@csc.fi (06/11/91)
Hello! Have anyone experience in cloning big (over 3kb inserts ) to Pgem -3Z or Pgem -4Z vectors (Promega)? I have difficulties to get 4kb insert into 2.7kb vector is this normal or has anyone else been noticed the same? What is the ratio between the sizes of vector and insert in ligation and also the concntration ratio? Yours Raimo Pollanen (M.S.) Pollanen@Csc.fi