collet@.scripps.edu (Thomas Collet) (06/12/91)
I am not sure whether a request similar to this has been posted recently, but in case it hasn't: We are interested in synthesizing our own oligos for PCR and sequencing/mutagenesis and are confused by the variety of machines offered. Our requirements are the following: * should be able to synthesize 2-4 oligos at a time * should be able to synthesize RNA * compatible with the new PAC phosphoamidites * require little maintenance and be easy to use * automatic monitoring of coupling efficiencies * sequencing grade product Also, we are interested in these OPC-type columns for the purification of the crude product. If you have had good - or bad - experiences with a par- ticular machine in your lab, please let me know. If there is enough interest, I will summarize and post on this bulletin board. Thomas COLLET@SCRIPPS.EDU COLLET@RISCSM.SCRIPPS.EDU