ew2f+@andrew.cmu.edu (Elaine M. Weidenhammer) (06/14/91)
Recently, two people in our lab have attempted HA-tagging using oligos; a third person is undertaking site-directed mutagenesis with an oligo. In at least two of the three cases, sequencing revealed mutations (insertions, rearrangements) in portions of the gene a considerable distance downstream of the site complementary to the oligo. Has anyone else had these difficulties? Does the source of the oligo make a difference? (i.e. a professional company vs. a university "part time" facility) Any comments are greatly appreciated! Elaine Weidenhammer