shapiro@mbcf.stjude.org (06/20/91)
We are currently performing a large number of fluroescent in situ hybridization (FISH) analyses of metaphase chromosomes using DNA prepared from EMBL3 phage clones. We have been preparing the DNA using Qiagen columns and have found lot to lot variability in the quality of the DNA for FISH, with significant background hybridization in some lots. We are interested in finding other rapid alternatives for high quality phage DNA preps (approximately 10-12 per week),such as modifications of DEAE cellulose minicolumns. We would like to avoid gradient purification of the DNAs or find some way to cleanup the Qiagen-prepared DNA to make it consistently suitable for FISH.